Sputum samples were thinned with sodium hydroxide for 10 min then treated with different concentrations of disinfectants for 20 min. After this time, phosphate buffer was added and the samples centrifuged at 4,000 x g for 20 min. The pellets were resuspended in 1 ml phosphate buffer and used to inoculate solid LJ and liquid MGIT culture tubes.
There was 100% agreement between the solid LJ and MGIT liquid culture results. The results are shown in the table below with the disinfectant and concentration used.
At the concentrations tested, ultraseptin appeared to be more effective than klenophen
for inactivation of TB in thinned sputum. In this study, the sputum was thinned with
sodium hydroxide prior to treatment in order to ensure that the disinfectant could
get access to the bacilli. It is not known whether the presence of sodium hydroxide
could have affected the disinfection making it more or less effective. This disinfection
study is part of our efforts to ensure that when culture of the extracted mycobacteria
is not required that our TB-
NOTE: the numbers of sputum samples tested were small and it should not be assumed that either disinfectant will inactivate all of the TB in every sample. In order to demonstrate this, a larger study should be performed. WORK SAFELY!
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To evaluate disinfectants (that are compatible with our TB-
At the concentrations tested, ultraseptin is more effective than klenophen (see table below).
Investigation of klenophen and ultraseptin for inactivation of TB in sputum
Consumption June 2011/1
+, growth of TB; -