Use of Seprion to identify abnormal aggregated proteins in Alzheimer’s Disease brain
Aggregated proteins were first extracted by Seprion followed by ELISA for the proteins of interest.
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Use of Seprion to monitor the development with age of ß-
Brains of mice at different ages were homogenised, extracted with Seprion and then tested in ELISA.
The increase of ß-
Use of Seprion to extract the abnormal prion protein from brain and spleen followed by an ELISA specific for the prion protein.
Seprion extracted the prion aggregates from multiple species and organs.
1. S. Wilson et al. 2004. A protein-
2. K. Sathasivam et al. Identical oligomeric and fibrillar structures captured from
the brains of R6/2 and knock-
3. L. A. Terry, et al. Detection of PrPsc in Blood from Sheep Infected with the Scrapie
and Bovine Spongiform Encephalopathy Agents. 2009. J. Virol. 83(23): 12552-
Use of Seprion to detect aggregated IgG and the influence of size-
Aggregated IgG was extracted using Seprion and then detected by ELISA.
Aggregates were detected but removed by filtration (300 KDa molecular weight cut-
3. Aggregated, not filtered
4. Aggregated, filtered
The Seprion ligand is a unique versatile tool that can be used to separate abnormal
aggregated proteins from the normal unaggregated protein (ref. 1) Seprion has been
used in the study of a wide range of protein aggregation diseases and in the monitoring
of aggregation in the production of therapeutic proteins. Proteins studied include:
aggregated therapeutic antibodies; huntingtin in Huntington’s Disease in animal models
(ref. 2); β-
See examples of the range of uses of Seprion below: